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bme (R&D Systems)

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R&D Systems bme
Bme, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bme/Cultrex+ReadyBME%2C+Stem+Cell+Qualified%2C+Reduced+Growth+Factor/pm42031998-58-6-7
Average 93 stars, based on 6 article reviews

bme - by Bioz Stars, 2026-10

93/100 stars

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Article Title: Interferon-γ selectively promotes survival of alveolar progenitor cells in a human lung organoid model.
Article Snippet: Cytokines IL-1A (Peprotech #200-01 A), IL-1B (BioLegend #579402), IL-6 (Peprotech #200-06), IL-33 (Peprotech #200-33), IL-4 (Peprotech #200-04), IL-10 (Peprotech #200-10), IL-13 (Peprotech #AF-200-13), IL-22 (Peprotech #200-22), and IFN-γ (Peprotech #300-02), were added to organoid cultures at a concentration of 10 ng/ml unless stated otherwise.

PDO:

Article Title: Accelerating personalized medicine: miniaturized patient-derived organoid drug screening for predicting cancer treatment responses and beyond
Article Snippet: .. PDO models were seeded in 70% BME (R&D systems, cat. no. 3434-050-RTU). .. Tumor PDOs were grown in a medium known as colon tumor medium (CTM), or complete medium, consisted of Advanced DMEM (Thermo Fisher Scientific, Waltham, MA, USA, cat. no 12491015) +/+/+ (Penicillin/Streptomycin 100 ng/mL, and Glutamax 1x, HEPES 100 mM) supplemented with 4% (v/v) Noggin (produced via the r-PEX protein expression platform at U-Protein Express BV), 1x B27 supplement (Life Technologies, cat. no. 17504-044), 1.25 mM N-acetyl-L-cysteine (Sigma-Aldrich, cat. no. A9165), 10 mM Nicotinamide (Sigma-Aldrich, cat. no. N0636), 50 ng/mL human EGF (PeproTech, cat. no. AF-100-15), 10 nM Gastrin (R&D, cat nr. 3006), 500 nM A83-01 (Tocris, cat. no 2939), 100 ng/mL, 250 ng/mL Rspondin-3 (Bio-techne, 3500-RS), and 50 μg/mL Primocin (InvivoGen, cat. no. ANT-PM-2).

Single Cell:

Article Title: Interferon-γ selectively promotes survival of alveolar progenitor cells in a human lung organoid model.
Article Snippet: DAPI (Sigma-Aldrich #10236276001), antiNGFR-PE (1:100; BioLegend #345106), anti-EPCAM-APC (1:100; BioLegend #369810), anti-CEACAM6-PE (1:100; Invitrogen #12- 0667-42), or isotype control-PE (1:100; Thermo Fisher Scientific #12-4714-82) were added, and cells were incubated on ice for 15–30 min. .. Cells were plated in a mixture of 20% washing buffer (Table EV1) and 80% BME (R&D Systems #3533-005-02) at 300–400 cells/ul as outlined in “Making single-cell suspensions and passaging organoids”. .. Cells were plated in a mixture of 20% washing buffer (Table EV1) and 80% BME (R&D Systems #3533-005-02) at 300–400 cells/ul as outlined in “Making single-cell suspensions and passaging organoids”.

Passaging:

Concentration Assay:

Article Title: Interferon-γ selectively promotes survival of alveolar progenitor cells in a human lung organoid model.
Article Snippet: .. When no BME was visible above the cell pellet anymore, the cells were resuspended in a mixture of 20% washing buffer (Table EV1) and 80% BME (R&D Systems #3533-005-02) at a concentration of 300–400 cells/μl. .. When no BME was visible above the cell pellet anymore, the cells were resuspended in a mixture of 20% washing buffer (Table EV1) and 80% BME (R&D Systems #3533-005-02) at a concentration of 300–400 cells/μl.

Cell Culture:

Article Title: Modeling herpes simplex virus type 2 and Zika virus replication in vaginal organoids and spheroids
Article Snippet: .. For organoid cultures, 20,000 cells were resuspended in 50 μl BME (R&D Systems Cat No. 3533-010-02) and cultured in DMEM/F12 supplemented with 2% Ultroser G (Sartorius Cat No. 15950-017), 1% PS, 50 ng/ml mouse EGF (Sigma Cat No. SRP3196-500UG), 0.5 mM SB431542 (Selleck Chemicals Cat No. S1067), and 10 mM Y-27632 (Tocris Cat No. 1254) (OCM). ..